HRZZ 9717 (2018-2022)


Sustainable production of biochemicals from waste lignocellulose containing feedstocks SPB-LCF; 9717

Host institution for the project:

Faculty of Food Technology and Biotechnology University of Zagreb

Principal Investigator: Božidar Šantek, PhD, Full Professor         Bibliography

Project summary:


This project proposal is dealing with development of sustainable bioprocesses for the biochemicals [microbial lipids (especially polyunsaturated fatty acids), L-lactate, 2,3-butanediol and polyphenols] production from the waste lignocellulose containing feedstocks (e.g. forestry residues, wheat straw, corn stover and cobs, waste grass, banana and beetroot peels, pea pods, onion peels, cocoa bean husks and walnut shells). Pretreatment of these feedstocks will be performed in high pressure reactor combined with the use of dilute acid or alkaline methods. In second stage of pretreatment, the solid residues (from high pressure reactor) will be hydrolyzed be using commercial enzymes mixtures or microorganism with relatively high cellulolytic activity. In this research, obtained hydrolyzates of lignocellulose containing feedstocks will be analyzed, corrected their content and used for biochemicals production. The study of biochemicals production will be focused on the semi-solid or solid state fermentations in the horizontal rotating tubular bioreactor (HRTB). The most suitable microorganisms for these biochemicals production will be identified and improved by genetic methods. Separation and purification procedure for studied biochemicals have to be developed and integrated with their production in order to improve ecological and economical aspects of these bioprocesses. The sustainability of these biochemicals production will be evaluated through the Life Cycle Assessment (LCA). The most efficient and sustainable bioprocess configuration will be defined on the basis of LCA results and consequently scale-up procedure will be developed. The scale-up procedure will be based on the developed integral bioprocess mathematical model and other scale-up criteria. Project outcomes will be new integrated bioprocesses for biochemicals production from waste lignocellulose containing feedstocks.





Project team

1. Predrag Horvat, PhD, Full Professor Bibliography
2. Vesna Zechner-Krpan, PhD, Full Professor Bibliography
3. Draženka Komes, PhD, Full Professor Bibliography
4. Ivan Krešimir Svetec, PhD, Full Professor Bibliography
5. Anita Slavica, PhD, Full Professor Bibliography
6. Tonči Rezić, PhD, Full Professor Bibliography
7. Mirela Ivančić Šantek, PhD, Full Professor Bibliography
8. Vlatka Petravić Tominac, PhD, Full Professor Bibliography
9. Jasna Mrvčić, PhD, Full Professor Bibliography
10. Sunčica Beluhan, PhD, Associate Professor Bibliography
11. Damir Stanzer, PhD, Associate Professor Bibliography
12. Antonija Trontel, PhD, Assistant Professor Bibliography
13. Mladen Pavlečić, PhD, Assistant Professor Bibliography
14. Mario Novak, PhD, Assistant Professor Bibliography
15. Anamarija Štafa, PhD, Assistant Professor Bibliography
16. Marina Svetec Miklenić, PhD, Assistant Professor Bibliography
17. Bojan Žunar, PhD Bibliography
18. Aleksandra Vojvodić Cebin, PhD Bibliography
19. Arijana Martinić, PhD Bibliography
20. Blanka Ljubas, PhD Bibliography
20. Martina Andlar, PhD Bibliography
21. Nenad Marđetko, PhD student Bibliography
22. Ana Huđek, PhD student Bibliography
23. Karla Hanousek-Čiča, PhD student Bibliography


Scientific project activities

Book Chapters

  1. Rezić, T., Trontel, A., Pavlečić, M, M. Novak, Herceg, Zoran, Ivančić M, Šantek, V. Petravić Tominac, Andlar, Martina, Rezić, I.  (2021) Nove spoznaje u biološkoj razgradnji lignoceluloznih sirovina. U: Šubarić, D. & Miličević, B. (ur.) Neke mogućnosti iskorištenja nusproizvoda prehrambene industrije. Osijek, Sveučilište Josipa Jurja Strossmayera u Osijeku, Prehrambeno-tehnološki fakultet Osijek, Veleučilište u Požegi, str. 1-25.
  2. Komes, D., Šeremet, D. (2019) Sekundarne biljne sirovine kao izvor funkcionalnih sastojaka u sustavu inkapsuliranih bioaktivnih formulacija, In: Neke mogućnosti iskorištenja nusproizvoda prehrambene industrije – Book 2., Ed. Drago Šubarić i Jurislav Babić, Josip Jurj Strossmayer University of Osijek Faculty of Food Technology Osijek, p. 15-36, ISBN: 978-953-7005-64-1

Scientific papers


  1. Šeremet, D.; Durgo, K.; Komljenović, A.; Antolić, M.; Mandura Jarić, A.; Huđek Turković, A.; Komes, D.; Šantek, B. Red Beetroot and Banana Peels as Value-Added Ingredients: Assessment of Biological Activity and Preparation of Functional Edible Films. Polymers 2022, 14, 4724.
  2. Ivušić, F.; Rezić, T.; Šantek, B. Heterotrophic Cultivation of Euglena gracilis in Stirred Tank Bioreactor: A Promising Bioprocess for Sustainable Paramylon Production. Molecules 2022, 27, 5866.
  3. Grubišić, M.; Mihajlovski, K.; Gruičić, A.M.; Beluhan, S.; Šantek, B.; Ivančić Šantek, M. Strategies for Improvement of Lipid Production by Yeast Trichosporon oleaginosus from
    Lignocellulosic Biomass. J. Fungi 2021, 7, 934.
  4.  Matanović, A.; Arambašić, K.; Žunar, B.; Štafa, A.; Svetec Miklenić, M.; Šantek, B.; Svetec, I.-K. Toolbox for Genetic Transformation of Non-Conventional Saccharomycotina Yeasts: High Efficiency Transformation of Yeasts Belonging to the Schwanniomyces Genus. J. Fungi 2022, 8, 531.
  5. Didak Ljubas, B., Novak, M., Trontel, A., Rajković, A., Kelemen, Z., Marđetko, N., Grubišić, M., Pavlečić, M., Petravić Tominac, V., Šantek, B. (2022) Production of Different Biochemicals by Paenibacillus polymyxa DSM 742 From Pretreated Brewers’ Spent Grains. Frontiers in Microbiology, 13, 812457, 16
  6. Grubišić, M., Galić Perečinec, M., Peremin, I., Mihajlovski, K., Beluhan, S., Šantek, B., Ivančić Šantek, M. (2022) Optimization of Pretreatment Conditions and Enzymatic Hydrolysis of Corn Cobs for Production of Microbial Lipids by Trichosporon oleaginosusEnergies, 15 (9), 3208, 16 doi:10.3390/en15093208.
  7. Pavlečić, M., Novak, M., Trontel, A., Marđetko, N., Grubišić, M., Didak Ljubas, B., Petravić Tominac, V., Čož Rakovac, R. & Šantek, B. (2022) Mathematical modelling of bioethanol production from raw sugar beet cossettes in a horizontal rotating tubular bioreactor. Fermentation, 8 (1), 13, 14 doi:10.3390/fermentation8010013.
  8. Ivančić Šantek, M., Grubišić, M., Galić Perečinec, M., Beluhan, S., Šantek, B. (2021) Lipid production by Mortierella isabellina from pretreated corn cobs and effect of lignocellulose derived inhibitors on growth and lipid synthesis. Process biochemistry (1991), 109, 46-58 doi:10.1016/j.procbio.2021.06.021.
  9. Andlar, M., Belskaya, H., Morzak Galina, Ivančić Šantek, M., Rezić, T., Petravić Tominac, V., Šantek, B. (2021) Biogas production systems and upgrading technologies: A review. Food Technology and Biotechnology, 59 (4), 387-412 doi:10.17113/ftb.
  10. Marđetko, N., Trontel, A., Novak, M., Pavlečić, M., Didak Ljubas, B., Grubišić, M., Petravić Tominac, V., Ludwig, R., Šantek, B. (2021) Screening of Lignocellulolytic Enzyme Activities in Fungal Species and Sequential Solid-State and Submerged Cultivation for the Production of Enzyme Cocktails. Polymers, 13 (3736), 3736, 16 doi:10.3390/ polym13213736.
  11. Osojnik Črnivec, I., Skrt, M., Šeremet, D., Sterniša, M., Farčnik, D., Štrumbelj, E., Poljanšek, A., Cebin, N., Pogačnik, L., Možina, S. (2021) Waste streams in onion production: Bioactive compounds, quercetin and use of antimicrobial and antioxidative properties. Waste management, 126, 476-486 doi:10.1016/j.wasman.2021.03.033.
  12.  Šeremet, D.,  Durgo, K., Jokić, S.,  Huđek, A., Vojvodić Cebin, A., Mandura, A.,  Jurasović, J.,   Komes, D. (2020) Valorization of Banana and Red Beetroot Peels: Determination of Basic Macrocomponent Composition, Application of Novel Extraction Methodology and Assessment of Biological Activity In Vitro. Sustainability, 12, 4539; doi:10.3390/su12114539
  13. Petravić-Tominac V., Nastav N., Buljubašić M., Šantek B. (2020) Current state of biogas production in Croatia. Energy, Sustainability and Society 10(1): 1-10.
  14. Žunar, B., Trontel, A.,  Svetec Miklenić, M.,  Prah, J.L., Štafa, A., Marđetko, N., Novak, M., Šantek, B., Svetec, I. K. (2020) Metabolically engineered Lactobacillus gasseri JCM 1131 as a novel producer of optically pure L- and D-lactate. World Journal of Microbiology and Biotechnology,  36(8):111. (
  15. Karača, S., Trifković, S., Martinić, A., Đorđević, V., Šeremet, D., Vojvodić Cebin, A., Bugarski, B., Komes, D. (2019) Development and characterization of functional cocoa (Theobroma cacao L.)-based edible films. International Journal of Food Science and Technology, doi:10.1111/ijfs.14407
  16. Šercer, M., Rezić, T.,  Godec, D.,  Oros, D.,  Pilipović, A.,  Ivušić, F., Rezić, I.,  Andlar, M., Ludwig, R., Šantek, B. (2019) Microreactor production by PolyJet Matrix 3D-printing technology: hydrodynamic characterization. Food Technology and Biotechnology, 57 (2), 272-281.
  17. Grubišić, M., Ivančić Šantek, M., Šantek, B. (2019) Potential of microalgae for the production of different biotechnological products. Chemical and Biochemical Engineering Quarterly, 33 (2), 161-181.


  1. Novak, M. ,  Trontel, A.,  Marđetko, N., Matoković, V.,  Sarić, M.,   Pavlečić, M.,  Šantek, B. (2020) Photoheterotrophic cultivation of purple non-sulfur bacterium Rhodovulum adriaticum on the media with different carbon sources. Croatian Journal of Food Technology, Biotechnology and Nutrition, 15 (3-4), 115-123.
  2.  Vojvodić Cebin, A., Šeremet, D.,  Mandura, A.,  Martinić, A.,  Komes, D. (2020) Onion Solid Waste as a Potential Source of Functional Food Ingredients. Engineering Power, 15(3), 7-13.


  1. Mrvčić, J., Srečec, S., Hanousek Čiča, K., Devčić, B., Petravić-Tominac, V., Trontel, A., Bosiljkov, T., Blažić, M., Čanadi Jurešić, G., Stanzer, D. (2022) Characterization of the Fermentation Process and Aroma Profile of Carob Brandy. U: Brka, M., Sarić, Z., Oručević Žuljević, S., Omanović-Mikličanin, E., Taljić, I., Biber, L. & Mujčinović, A. (ur.)10th Central European Congress on Food-Proceedings of CE-Food 2021 doi:10.1007/978-3-031-04797-8_7, pp 68-87.
  2. Šeremet, D., Mandura, A., Vojvodić Cebin, A., Jokić, S., Komes, D. (2020) Development and characterization of dopamine-loaded liposomes extracted from banana peel, Proceedings of 18 international conference Ružička Days Today Science – Tomorrow , 16-18. 09. 2020., Vukovar, Croatia, pp. 132-140.


B) Participation in scientific conferences

a) Invited lectures:

  1. T. Rezić, B. Šantek (2021) From the PDE model to the model of casualty: Example of nanocellulose production,  Conference of Swiss Federal Institute of Technology, Lausanne, Switzerland, 01-04. 11.2021.

b) Lectures:

  1. Didak Ljubas, B., Novak, M., Trontel, A., Kelemen, Z., Marđetko, N., Grubišić, M., Pavlečić, M., Petravić Tominac, V., Šantek, B. (2022) Paenibacillus polymyxa DSM 742 – the future of sustainable production of 2,3-butanediol. In: 7th Croatian Congress of Microbiology with International Participation – Book of Abstracts (lecture), 24-27.5.2022.
  2. Matanović, A., Svetec Miklenić, M,, Štafa, A., Šantek, B., Svetec, I.K. (2022) PRODUCTION OF L-LACTATE AND 2,3-BUTANEDIOL USING GENETICALLY MODIFIED YEAST Kluyveromyces marxianus In: 19th Ružička Days “Today Science – Tomorrow Industry” Vukovar, Hrvatska, 2022. str. 17-17 (lecture)
  3. Štafa, Anamarija; Matanović, Angela; Svetec Miklenić, Marina; Šantek, Božidar; Svetec, Ivan Krešimir (2022) Gene targeting efficiency in non-conventional yeasts In: FEMS Conference on Microbiology 2022, Beograd, Srbija, 2022. str. 79-79

Poster presentations:

  1. Marđetko, N., Trontel, A., Novak, M., Pavlečić, M., Didak Ljubas, B., Grubišić, M., Petravić Tominac, V., Ludwig, R., Šantek, B. (2022) Sustainable Production of Fungal Lignocellulolytic Enzyme Cocktails. U: 7th Croatian Congress of Microbiology with International Participation – Book of Abstracts. 116-116.
  2. Svetec Miklenić, M., Matanović, A., Slišković, A., Štafa, A.; Šantek, B., Svetec, I.K. (2022) Genetic transformation of non-conventional yeasts belonging to the Schwanniomyces genus. U: 7th Croatian Congress of Microbiology with International Participation, Sveti Martin na Muri, Hrvatska, 2022. pp 121-121.
  3. Šeremet, D., Mandura, A., Vojvodić Cebin, A., Jokić, S., Komes, D. (2020) Development and characterization of dopamine-loaded liposomes extracted from banana peel. 18 international conference Ružička Day Today Science Tomorrow Industry, 16.- 18.09.2020, Hrvatska, pp. 117.
  4.  Trontel, A., Novak, M., Pavlečić, M., Marđetko, N., Mrak, K., Lončar, A., Šantek, B. (2020) Selection of non-Saccharomyces yeast with tolerance to high lactic acid concentrations for production of biochemical. Microbial Stress 2020 online: European Federation of Biotechnology,  16.-18.10.2020, pp. 61-61. (on-line konferencija)
  5. Novak, M., Pavlečić, M., Marđetko, N., Trontel, A., Madaras, M., Sarić, M., Šantek, B. (2020) Kinetic analysis of purple non-sulphur bacteria Rhodovulum adriaticum DSM 2781 growth on carbon sources from lignocellulosic hydrolysates. Microbial Stress 2020 online: European Federation of Biotechnology, 16.-18.10.2020, pp. 60-60. (on-line konferencija)
  6. Pavlečić, M., Kralj, M., Trontel, A., Novak, M., Grubišić, M., Marđetko, N., Šantek, B. (2020) The influence of temperature on specific growth rate and ethanol production for Kluyveromyces marxianus NBRC 1777. Microbial Stress 2020 online: European Federation of Biotechnology,  16.-18.10.2020, pp. 63-63. (on-line konferencija)
  7. Matanović, A., Svetec Miklenić, M., Šantek, B., Svetec, I.K. (2020) Efficient transformation procedure for yeasts belonging to the Schwanniomyces genus. FEMS Online Conference on Microbiology, 28.-31.10.2020.
  8. Marđetko, N., Trontel, A., Novak, M., Šantek, B., (2019) Biotechnological production of xylitol from lignocellulosic biomass hydrolysates. Powers of Microbes in Industry and Environment 2019, 15.-18. 05. 2019., Sveti Martin na Muri, Hrvatska, pp. 94
  9. Poklar Ulrih, N., Skrt, M., Osojnik Črnivec, I.G., Šeremet, D., Komes, D. (2019)     Proliposomal encapsulation of quercetin extracts from onion peel. 8th International   Symposium on „Delivery of Functionality in Complex Food System”,07.-10.07.2019., Porto, Portugal, pp. 82
  10. Mandura, A., Šeremet, D., Vojvodić Cebin, A., Martinić, A.,  Šantek, B., Komes, D. (2019) Application of banana peel extract in the formulation of functional edible films. 12th International Scientific and Professional Conference „With Food to Health“, 24.-25.10.2019., Osijek, Hrvatska, pp. 68
  11. Šeremet, D., Huđek, A., Durgo, K., Mandura, A., Vojvodić Cebin, A., Martinić, A., Šantek, B., Komes, D. (2019) The red beetroot peel extract – characterization of bioactive composition and biological activity in vitro12th International Scientific and Professional Conference „With Food to Health“, 24.-25.10.2019., Osijek, Hrvatska, pp. 59.


Doctoral thesis

  1. Marđetko, N. (2022) ‘Development of a bio-refinery system for biofuels and bichemicals production from waste lignocellulose containing feedstocks’, doctoral thesis, Faculty of food Technology and Biotechnology, Zagreb.

Science popularization

  1. Beluhan, S., Ivančić-Šantek, M., Šantek, B. (2022) Proizvodnja kombuche i bakterijske nanoceluloze iz povrtnih ostataka. ARCA 2022- 20th International Innovation Exibition . 13-15 October 2022, Zagreb, Croatia. SBeluhan-et-al-ARCA-2022
  2. Dan otvorenih vrata Prehrambeno-biotehnološkog fakulteta, Pierottijeva 6, 23. travnja 2022. (
    Radionica 1: Proizvodnja biogoriva i biokemikalija iz obnovljivih sirovina“ ( Mladen Pavlečić, doc. Antonija Trontel, Blanka Ljubas)
    Radionica 2: Proizvodnja piva ( Mario Novak, Nenad Marđetko,, Marina Grubišić
  3. Festival -znanosti 2021.Tehnički muzej „Nikola Tesla“, Savska cesta 18

    Kako kulturno iskoristiti obnovljive sirovine u biotehnološkim procesima?
    Šareni svijet biotehnologije; Lignocelulozni otpad kao obnovljive sirovine u biotehnologiji;
    Bioplin proizveden iz obnovljivih sirovina; Bioplastika – što je i kako se proizvodi?;
    Biotehnološka primjena mikroalgi; voditelji: Vlatka Petravić Tominac, Božidar Šantek, Tonči Rezić, Mirela Ivančić Šantek, Sunčica Beluhan, Antonija Trontel, Mario Novak, Mladen Pavlečić, Nenad Marđetko, Marina Grubišić i Blanka Ljubas (festival znanosti 2021).

  4. Ivančić Šantek, M., Grubišić, M., Beluhan, S., Šantek, B., Microbial lipids – feedstock for biodiesel production, 18. međunarodna izložba inovacija ARCA 2020, 15.- 17.10.2020, Zagreb, Hrvatska, srebrena medalja (mivancic-santek-et-al-ARCA)
  5. A. Matanović, M. Svetec Miklenić, B. Žunar, A. Štafa, I.K. Svetec, Genetic transformation of  yeasts from genus SchwanniomycesARCA 2019. 17th edition of the International Exhibition of Inventions, 17.-19.10. 2019., Zagreb, Hrvatska, bronze medal (Svetec-IK-ARCA-2019)